Supplementary data for Johnson et al., 2004
Full alignments:
Troponin I
Synaptotagmin
Alpha-Tubulin
Noto-9
Forkhead
Snail
Tropomyosin-like
Brachyury
For TnI, Syt, Tub, and Noto9, first line is savignyi sequence, the second line is intestinalis sequence,
the third line is all the ESTs from the region.
For Fkh, Snail, Tro-like, and Bra, first line is intestinalis sequence, the second line is est sequence,
the third line is savignyi.
Sequences and photos for constructs used in this study:
Note: photos not included for nonfunctional constructs.
Pro constructs:
Synaptotagmin photo
Alpha-Tubulin photo
Noto9 photo
Eight electroporations, same dechorionation, four DNA preps (pro.1793), shows variability in mosaicism:
1
2
3
4
5
6
7
8
Troponin Pro constructs:
pro.1793 photo
pro.d.509.338 photo
pro.d.535.861 photo
pro.d.535.1160 photo
pro.1591 photo
pro.1536 photo
pro.1464
pro.ci.921 photo
Msrr constructs:
msrr.363 photo
msrr.363.d.fkhpro photo
msrr.363.reverse
msrr.363.reverse.d.fkhpro
msrr.suff.132 photo
msrr.399.d.chaos.1 photo
msrr.399.d.chaos.2 photo
msrr.399.d.chaos.3 photo
msrr.399.d.chaos.4 photo
msrr.399 photo
msrr.352
msrr.301
msrr.98
msrr.221
msrr.d.47.97
msrr.d.127.106 photo
msrr.d.206.100
Troponin Scrambled window consructs:
Window 1 photo
Window 2 photo
Reverted Window 2 photo
Window 3 photo
Window 4 photo
Window 5 photo
Window 6 photo
Window 7 photo
Window 8 photo
Window 9 photo
Window 10 photo
Window 11 photo
Window 12 photo
Window 13 photo
Window 14 photo
Window 15 photo
Window 16 photo
Window 17 photo
Window 18 photo
Window 19 photo
Window 20 photo
Base plasmids:
djmcs.lacZ
pCES
Perl code:
Deletion_picker.pl
Profiler.pl
Primer_picker.pl
PSSM_maker.pl
Scanning_mutagenesis_picker.pl
DNA_scanner.pl