Position Mutant Score Untreated Frequency Treated Frequency Rank if any 2 H 8.4 0.0 0.2 0 2 K 7.1 0.3 0.0 0 3 N 8.2 0.2 0.1 0 3 V 6.2 0.0 0.1 0 4 S 1.8 0.1 0.0 0 5 F 12.6 0.1 0.2 0 6 C 25.4 0.0 0.1 0 6 L 33.2 0.0 0.1 0 7 H 7.4 0.1 0.0 0 8 G 25.9 0.0 0.1 0 8 L 19.0 0.1 0.0 0 8 Q 14.4 0.1 0.1 0 10 F 5.7 0.2 3.9 0 10 I 1.1 9.1 37.4 0 10 M 7.7 0.1 0.0 0 10 R 36.6 0.0 0.9 0 10 S 10.5 0.1 0.0 0 10 V 4.5 3.5 4.3 0 10 Y 10.3 0.0 0.1 0 11 A 5.9 0.1 0.1 0 11 F 4.1 0.1 0.0 0 11 I 1.7 0.4 0.9 0 12 A 5.0 2.6 1.1 0 12 E 3.2 0.3 0.5 0 12 I 6.7 0.9 0.7 0 12 K 2.9 1.0 0.5 0 12 N 2.1 0.7 0.5 0 12 P 4.6 1.8 1.8 0 12 Q 2.4 0.0 0.1 0 12 R 2.9 0.1 0.0 0 12 S 1.9 7.8 3.4 0 12 V 6.1 0.0 0.1 0 13 A 3.8 0.6 0.0 0 13 L 3.6 0.1 0.1 0 13 V 1.5 35.8 19.5 0 14 E 6.1 0.3 0.1 0 14 M 3.6 0.1 0.0 0 14 N 2.4 0.1 0.0 0 14 Q 2.4 0.1 0.0 0 14 R 3.1 19.7 9.7 0 14 T 3.2 0.2 0.0 0 15 K 27.3 0.1 0.0 0 15 L 5.4 0.4 0.3 0 15 M 11.6 0.1 0.1 0 15 V 4.5 23.0 19.3 0 16 A 4.2 2.0 0.8 0 16 E 2.0 9.4 2.3 0 16 R 5.5 0.2 0.0 0 17 D 8.0 0.3 0.1 0 17 E 9.2 2.0 0.4 0 18 E 8.0 0.2 0.1 0 18 H 3.0 0.3 1.2 0 18 K 7.3 0.1 0.0 0 18 L 4.9 0.5 0.4 0 18 R 8.3 0.1 0.0 0 19 I 2.4 9.8 7.7 0 19 M 3.6 0.3 0.0 0 19 P 1.4 1.8 0.2 0 19 Q 5.4 1.0 1.1 0 19 S 3.1 0.2 0.1 0 19 T 3.3 1.0 0.6 0 19 V 2.2 1.7 1.0 0 20 I 2.1 14.4 5.8 0 20 L 4.7 0.0 0.1 0 20 M 5.8 0.2 2.3 0 20 R 5.3 5.8 8.6 0 20 T 3.7 0.0 1.8 0 20 V 1.4 0.1 0.3 0 21 G 9.9 0.1 0.0 0 21 K 2.4 0.2 0.0 0 22 P 15.4 0.1 0.0 0 22 T 7.1 0.2 0.0 0 22 V 4.2 0.1 0.5 0 23 I 15.5 0.0 0.7 0 23 V 25.8 0.0 0.1 0 24 F 12.6 0.0 0.2 0 24 I 15.5 0.0 5.8 35 30 N 3.5 0.0 8.7 24 31 P 30.9 0.0 0.1 0 32 I 1.6 0.0 3.6 20 33 F 5.9 0.4 3.0 0 33 I 0.8 0.4 1.8 0 33 M 9.3 0.0 0.1 0 33 V 4.2 1.3 0.8 0 34 A 3.0 0.3 0.0 0 34 D 4.3 0.0 0.4 0 34 G 2.9 0.4 0.0 0 34 K 2.6 0.2 0.3 0 34 Q 1.6 0.0 0.6 0 35 D 3.0 34.3 30.8 0 35 G 2.9 0.2 0.5 0 35 K 2.9 0.3 0.0 0 35 N 2.5 0.9 0.1 0 35 Q 2.4 0.4 0.0 0 36 I 1.2 48.9 29.9 0 36 L 1.8 0.8 0.8 0 36 T 3.3 0.2 0.0 0 36 V 2.4 1.1 1.5 0 37 A 18.6 0.5 0.4 0 37 C 19.9 0.7 0.8 0 37 D 2.3 9.6 14.8 0 37 E 1.9 1.0 2.6 0 37 H 4.5 1.1 1.2 0 37 I 28.3 0.1 0.2 0 37 K 3.3 2.9 0.2 0 37 P 10.0 0.0 0.1 0 37 Q 3.8 0.1 0.1 0 37 T 7.0 1.8 2.9 0 37 Y 7.0 0.0 0.4 0 38 F 8.2 0.3 0.3 0 38 I 13.7 0.2 0.0 0 38 W 6.7 0.0 0.1 0 39 L 6.9 0.2 0.0 0 39 Q 3.1 0.6 0.4 0 39 S 2.0 1.9 1.3 0 39 T 3.3 0.3 0.1 0 40 R 8.7 0.2 0.0 0 41 I 3.9 0.1 0.0 0 41 K 2.1 53.5 22.6 0 41 N 1.0 2.7 0.0 0 43 N 3.9 0.1 0.5 0 43 Q 2.6 0.0 0.1 0 43 R 1.7 2.4 1.8 0 43 T 5.4 0.1 2.3 0 45 Q 4.3 0.0 0.4 0 45 R 3.0 1.6 2.6 0 46 I 2.1 0.2 19.9 11 46 L 3.0 0.3 10.3 14 46 V 6.7 0.0 0.3 18 47 M 15.1 0.1 0.0 0 47 V 3.2 0.1 1.3 22 48 V 10.3 0.1 5.8 9 50 L 10.4 0.0 0.2 27 50 M 15.7 0.0 0.1 0 50 V 10.7 0.0 0.6 19 51 A 22.1 0.0 0.1 0 51 K 29.1 0.0 0.1 0 51 R 36.5 0.2 0.0 0 52 S 7.5 0.1 0.0 0 53 I 2.5 0.0 0.1 0 53 L 2.9 0.0 4.3 28 53 Y 2.9 0.0 0.2 36 54 A 21.9 0.0 0.3 26 54 L 3.5 0.0 1.1 13 54 M 10.9 0.0 0.8 12 54 S 25.6 0.0 0.1 16 54 T 21.9 0.1 1.3 17 54 V 10.1 0.0 21.0 15 55 E 3.6 0.1 0.0 0 55 N 1.4 0.0 0.1 0 55 R 3.3 0.1 3.0 0 57 K 1.3 12.4 11.0 0 57 S 13.9 0.0 0.1 0 58 E 2.0 0.1 3.8 0 59 F 5.5 0.1 0.0 0 60 E 4.7 6.4 8.3 0 60 N 2.3 0.0 0.1 0 61 D 3.3 0.7 0.3 0 61 E 4.9 2.5 2.9 0 61 H 5.0 0.6 0.6 0 61 K 3.9 0.1 0.0 0 61 N 1.1 2.5 1.4 0 61 S 4.0 0.1 0.0 0 62 M 6.4 0.4 0.2 0 62 V 1.9 12.3 30.2 0 63 A 4.5 2.5 3.1 0 63 C 4.3 2.7 1.3 0 63 E 1.5 0.0 0.1 0 63 H 2.5 1.6 1.3 0 63 I 3.3 0.6 0.1 0 63 M 2.4 0.1 0.0 0 63 N 2.4 0.2 0.0 0 63 P 4.7 34.9 71.5 0 63 Q 1.6 1.0 2.2 0 63 R 3.5 0.2 0.0 0 63 S 4.4 4.0 2.9 0 63 T 4.1 3.4 2.2 0 63 V 4.2 1.6 0.5 0 64 L 10.4 2.4 2.1 0 64 M 14.8 2.5 1.3 0 64 V 2.9 13.0 18.9 0 65 D 6.9 2.3 2.1 0 65 G 4.4 0.1 0.0 0 65 K 2.7 0.0 0.1 0 66 F 7.5 0.0 1.0 0 66 L 4.7 0.0 0.2 0 66 V 3.5 0.0 0.4 0 67 D 2.7 0.3 0.4 0 67 E 2.5 1.4 0.0 0 67 F 2.7 0.0 0.8 0 67 G 4.5 0.2 0.0 0 67 H 2.3 0.1 0.0 0 67 S 3.4 0.8 0.7 0 67 W 4.7 0.1 0.0 0 67 Y 3.5 0.7 0.8 0 68 E 7.0 0.3 0.2 0 69 I 20.4 0.0 0.1 0 69 K 1.0 38.1 4.7 0 69 N 7.6 0.3 0.7 0 69 Q 5.7 3.3 2.3 0 69 R 2.0 0.4 0.5 0 69 Y 11.2 2.1 1.5 0 70 E 4.4 0.5 0.1 0 70 Q 2.0 0.2 0.6 0 70 R 1.7 7.7 2.6 0 70 T 3.4 0.2 0.4 0 71 G 4.9 0.0 0.1 0 71 I 1.7 0.0 1.3 0 71 L 3.3 0.0 0.1 0 71 T 3.9 2.5 9.4 0 71 V 1.6 1.3 29.9 34 72 E 6.1 0.3 0.9 0 72 K 2.0 0.0 0.1 0 72 L 2.2 0.4 1.7 0 72 M 2.8 1.0 1.4 0 72 R 2.0 0.1 0.4 0 72 T 4.8 2.1 3.2 0 72 V 4.0 3.9 8.1 0 73 A 4.0 0.0 0.4 30 73 C 19.2 0.0 1.4 31 73 S 7.2 0.0 10.4 32 73 T 18.6 0.0 1.7 33 73 V 28.2 0.0 0.1 0 74 A 25.2 0.4 1.2 0 74 P 31.4 0.0 0.9 0 74 S 2.7 0.8 4.5 0 75 I 2.6 0.3 0.6 0 76 V 24.7 0.0 1.4 0 77 I 2.0 15.7 32.4 0 77 L 5.2 0.1 0.0 0 78 R 36.5 0.2 0.0 0 79 A 3.6 0.0 0.5 0 79 D 2.9 0.0 0.2 0 79 H 4.7 0.1 0.0 0 79 L 7.2 0.0 0.1 0 79 S 4.0 0.0 0.1 0 82 A 18.8 0.2 25.0 4 82 C 5.6 0.0 0.1 0 82 F 8.9 0.0 1.8 5 82 I 1.8 3.0 1.7 37 82 L 10.4 0.0 0.1 0 82 S 13.1 0.0 0.3 7 82 T 13.6 0.0 2.9 6 83 D 6.3 0.0 0.3 0 83 Y 19.0 0.1 0.0 0 84 V 10.7 0.0 12.2 1 85 M 7.8 0.1 0.0 0 85 V 6.6 0.0 3.1 0 86 R 36.5 0.2 0.0 0 87 G 25.9 0.1 0.0 0 87 K 6.1 0.1 0.1 0 88 D 5.8 0.1 5.4 23 88 G 17.9 0.0 0.1 0 88 S 11.6 0.0 2.0 29 88 T 13.6 0.0 0.2 25 89 I 1.4 0.8 0.5 0 89 M 6.2 38.7 3.5 0 89 T 7.3 0.0 0.1 0 89 V 4.3 0.1 1.0 0 90 F 11.6 0.1 0.0 0 90 M 7.5 0.2 34.3 3 91 A 4.4 0.1 0.1 0 91 P 6.7 0.1 0.0 0 91 V 4.8 0.1 0.0 0 92 H 3.8 0.2 0.3 0 92 K 4.0 0.4 1.4 0 92 R 5.6 0.0 0.9 0 93 L 0.9 26.9 37.0 0 93 M 5.5 0.1 0.4 0 93 V 6.9 0.1 0.0 0 94 S 7.5 0.1 0.0 0 95 F 2.4 0.0 1.4 0 95 R 28.8 0.0 0.1 0 95 S 3.9 3.5 0.0 0 95 W 5.9 0.1 0.0 0 96 S 1.8 0.0 1.2 0 98 I 7.4 0.4 0.0 0 98 K 24.3 0.4 0.0 0 99 L 12.8 0.3 0.2 0