Wild Type Position Mutant Gap Weight Score Prediction Phenotype Alignment S 80 P 0 5.54271 Intermediate Pyruvate Kinase Deficiency SSSSSSSSSSSSEESSMMMDLDTATTTFAF R 86 P 0 40.696 Negative Pyruvate Kinase Deficiency RRRRRRRRRRRRRRRRRRRRRRRRRRRRRR I 90 N 0 15.7969 Negative Pyruvate Kinase Deficiency IIIIIIIIIIIIIVIIIIIIIIIIIIIIII G 95 R 0 36.5233 Negative Pyruvate Kinase Deficiency GGGGGGGGGGGGGGGGGGGGGGGGGGGGGG M 107 T 0 13.3068 Negative Pyruvate Kinase Deficiency MMMMMMMMMMMMLMMMLLLLLVLLLLLLLL G 111 R 0 36.5233 Negative Pyruvate Kinase Deficiency GGGGGGGGGGGGGGGGGGGGGGGGGGGGGG A 115 P 0 13.459 Negative Pyruvate Kinase Deficiency AAAAAAAAAAAAAAAAVVVVVVIVVVIAVV S 120 F 0 21.5696 Negative Pyruvate Kinase Deficiency SSSSSSSSSSSSSSSSSSSSSSSSSSSSSS S 130 Y 0 6.21837 Intermediate Pyruvate Kinase Deficiency STSTSTSTTTTTTTTTVVVVVVVVVVVVVV V 134 D 0 36.4342 Negative Pyruvate Kinase Deficiency VVIVIVIVVVVVVIVIAAAAATAAAAAVAA I 153 T 0 15.084 Negative Pyruvate Kinase Deficiency IVIVIVIVIIIVIIIIIIIIIIIIIIIIII L 155 P 0 36.3167 Negative Pyruvate Kinase Deficiency LLLLLLLLLLLLLLLLLLLLLLLLLLLLLL G 159 V 0 29.3626 Negative Pyruvate Kinase Deficiency GGGGGGGGGGGGGGGGGGGGGGGGGGGGGG R 163 C 0 29.0061 Negative Pyruvate Kinase Deficiency RRRRRRRRRRRRRRRRRRRRRRRRRRRRRR E 172 Q 0 2.65744 Positive Pyruvate Kinase Deficiency ETETETETTTTTSMTTDDDDGGDDDEDDED I 219 T 0 13.5345 Negative Pyruvate Kinase Deficiency IVIVIVIVIVIVIIVVVVVVVIVVVVVVVV G 222 A 0 22.0523 Negative Pyruvate Kinase Deficiency GGGGGGGGGGGGGGGGGGGGGGGGGGGGGG G 263 R 0 34.2435 Negative Pyruvate Kinase Deficiency GAGAGAGAAAAAAAAAAAAAAPPAAAPAAA G 263 W 0 12.0363 Negative Pyruvate Kinase Deficiency GAGAGAGAAAAAAAAAAAAAAPPAAAPAAA G 275 R 0 36.5233 Negative Pyruvate Kinase Deficiency GGGGGGGGGGGGGGGGGGGGGGGGGGGGGG D 281 N 0 3.12715 Positive Pyruvate Kinase Deficiency DDDDDDDDDDDDDDDDDDDDDDHHHHHDHD F 287 V 0 17.6108 Negative Pyruvate Kinase Deficiency FFFFFFFFFFFFFFFFFFFFFFFFFFFFFF V 288 L 0 10.167 Negative Pyruvate Kinase Deficiency VIVIVIVIIIIIIIIIIIIVIIIIIIIIII D 293 N 0 2.60172 Positive Pyruvate Kinase Deficiency DDDDDDDDDDDDGGAADDDDDDDDDDDDDD A 295 V 0 5.11996 Positive Pyruvate Kinase Deficiency AHVHLHAHHHQHRHTKRRRQIRLLLLLKLK I 310 N 0 15.8612 Negative Pyruvate Kinase Deficiency IIIIIIIIIIIIIIIIIIIVIIIIIIIIII I 314 T 0 21.4442 Negative Pyruvate Kinase Deficiency IIIIIIIILLLIIIIIIIIIIIIIIIIIII E 315 K 0 19.981 Negative Pyruvate Kinase Deficiency EEEEEEEEEEEEEEEEEEEEEEEEEEEEEE D 331 E 0 23.9774 Negative Pyruvate Kinase Deficiency DDDDDDDDDDDDDDDDDDDDDDDDDDDDDD D 331 N 0 13.9665 Negative Pyruvate Kinase Deficiency DDDDDDDDDDDDDDDDDDDDDDDDDDDDDD G 332 S 0 6.88687 Intermediate Pyruvate Kinase Deficiency GGGGGGGGGGGGGGGGGGGGGGGGGGGGAG V 335 M 0 17.6092 Negative Pyruvate Kinase Deficiency VVMVVVVVVVVVVVVVVVVVVVIVVVIVVV A 336 S 0 23.3907 Negative Pyruvate Kinase Deficiency AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA R 337 P 0 40.696 Negative Pyruvate Kinase Deficiency RRRRRRRRRRRRRRRRRRRRRRRRRRRRRR R 337 Q 0 14.4345 Negative Pyruvate Kinase Deficiency RRRRRRRRRRRRRRRRRRRRRRRRRRRRRR D 339 H 0 20.3174 Negative Pyruvate Kinase Deficiency DDDDDDDDDDDDDDDDDDDDDDDDDDDDDD G 341 A 0 22.0523 Negative Pyruvate Kinase Deficiency GGGGGGGGGGGGGGGGGGGGGGGGGGGGGG G 341 D 0 35.8645 Negative Pyruvate Kinase Deficiency GGGGGGGGGGGGGGGGGGGGGGGGGGGGGG I 342 F 0 9.95841 Negative Pyruvate Kinase Deficiency IIITIIIIIIIIIIIIIIIIIIIIIIIIII K 348 N 0 7.34216 Intermediate Pyruvate Kinase Deficiency KKKKKKKKKKKKKKKKKKKQQQEEQQEEEE A 352 D 0 40.3336 Negative Pyruvate Kinase Deficiency AAASAAAAAAAAAAAAAAAAAAIVVVIAVA I 357 T 0 8.76708 Negative Pyruvate Kinase Deficiency IIIIIIIIITIIIIIIIIIIIIIIIIIIII R 359 C 0 19.1143 Negative Pyruvate Kinase Deficiency RRRRRRRRRKRRKKRRKKKKKKKKKKKMKM R 359 H 0 7.40098 Intermediate Pyruvate Kinase Deficiency RRRRRRRRRKRRKKRRKKKKKKKKKKKMKM N 361 D 0 29.2717 Negative Pyruvate Kinase Deficiency NNNNNNNNNNNNNNNNNNNNNNNNNNNNNN G 364 D 0 35.8645 Negative Pyruvate Kinase Deficiency GGGGGGGGGGGGGGGGGGGGGGGGGGGGGG V 368 F 0 4.43473 Positive Pyruvate Kinase Deficiency VIVIVIVITIIIIIIIIIIIAIIIIIIIII S 376 I 0 21.6713 Negative Pyruvate Kinase Deficiency SSSISSSSSSSSSSSSSSSSSSSSSSSSSS T 384 M 0 29.4988 Negative Pyruvate Kinase Deficiency TTTTTTTTTTTTTTTTTTTTTTTTTTTTTT E 387 G 0 28.2249 Negative Pyruvate Kinase Deficiency EEEEEEEEEEEEEEEEEEEEEEEEEEEEEE D 390 N 0 13.9665 Negative Pyruvate Kinase Deficiency DDDDDDDDDDDDDDDDDDDDDDDDDDDDDD A 392 T 0 20.0874 Negative Pyruvate Kinase Deficiency AAAAAAAAAAAAAAAAAAAGGAGGGGGGGG N 393 K 0 25.31 Negative Pyruvate Kinase Deficiency NNNNNNNNNNNNNNNNNNNNNNNNNNNNNN N 393 S 0 13.4036 Negative Pyruvate Kinase Deficiency NNNNNNNNNNNNNNNNNNNNNNNNNNNNNN A 394 D 0 43.1517 Negative Pyruvate Kinase Deficiency AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA A 394 V 0 35.488 Negative Pyruvate Kinase Deficiency AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA T 408 A 0 37.9905 Negative Pyruvate Kinase Deficiency TTTTTTTTTTTTTTTTTTTTTTTTTTTTTT T 408 I 0 33.4841 Negative Pyruvate Kinase Deficiency TTTTTTTTTTTTTTTTTTTTTTTTTTTTTT Q 421 K 0 23.2293 Negative Pyruvate Kinase Deficiency QQQQQQQQQQQQMMMMMMMMMMMMMMMMMM R 426 Q 0 4.02658 Positive Pyruvate Kinase Deficiency RRRRRRRRRRRRKKKKLLLLRLLVLLLLLL R 426 W 0 11.3998 Negative Pyruvate Kinase Deficiency RRRRRRRRRRRRKKKKLLLLRLLVLLLLLL E 427 A 0 5.33232 Intermediate Pyruvate Kinase Deficiency EEEEEEEEEEEEEEEELLLVVLIIIIIKIK E 427 D 0 3.73732 Positive Pyruvate Kinase Deficiency EEEEEEEEEEEEEEEELLLVVLIIIIIKIK A 431 T 0 7.34727 Intermediate Pyruvate Kinase Deficiency AAAAAAAAAAAAAAAAAAAASATAAATTAT G 458 D 0 39.6294 Negative Pyruvate Kinase Deficiency GGGGGGAGGGGGAAAAAAASAAASAAAASA A 459 V 0 13.0793 Negative Pyruvate Kinase Deficiency AAAAAATAAAAAAAAGAAAAAAAASAAAAS V 460 M 0 8.39622 Negative Pyruvate Kinase Deficiency VVVVVVVVVVVVTVVAVVVVIVTVVVTVVV A 468 G 0 5.73289 Intermediate Pyruvate Kinase Deficiency ASASASASAAASAAAAAAAAAAGAAAGAAA A 468 V 0 22.5776 Negative Pyruvate Kinase Deficiency ASASASASAAASAAAAAAAAAAGAAAGAAA T 477 A 0 23.4517 Negative Pyruvate Kinase Deficiency TSTSTSTSTTTSTTSSSSSSSSTSTTTSSS R 479 H 0 2.2656 Positive Pyruvate Kinase Deficiency RRRRRRRRRRRRRKKRNKNTNENTDDNDTE S 485 F 0 17.2436 Negative Pyruvate Kinase Deficiency SASASASSSSSASSSSSSSSSSSSASSSSS R 486 W 0 16.7126 Negative Pyruvate Kinase Deficiency RRRRQRRRRRRRRRKKKKKKKKKKKKKKKK R 488 Q 0 11.4956 Negative Pyruvate Kinase Deficiency RRRRRRRRRRRRKRRRRRRRRRRRKKRRRR R 490 W 0 3.71609 Positive Pyruvate Kinase Deficiency RRRRRRRRRRRRAPRRVVVNSKSNNNSVDV A 495 T 0 5.17309 Positive Pyruvate Kinase Deficiency AAAAAAASAAAATTAAMMMMMTLLMMLMLM A 495 V 0 5.6285 Intermediate Pyruvate Kinase Deficiency AAAAAAASAAAATTAAMMMMMTLLMMLMLM R 498 C 0 29.0061 Negative Pyruvate Kinase Deficiency RRRRRRRRRRRRRRRRRRRRRRRRRRRRRR R 498 H 0 14.4067 Negative Pyruvate Kinase Deficiency RRRRRRRRRRRRRRRRRRRRRRRRRRRRRR R 504 L 0 19.0324 Negative Pyruvate Kinase Deficiency RRRRRRRRRRRRRRRRRRRRRRRRRRRRRR V 506 I 0 2.45309 Positive Pyruvate Kinase Deficiency VAVAVAAAAAAALLACSSSSSIASSSASSA R 510 Q 0 14.4345 Negative Pyruvate Kinase Deficiency RRRRRRRRRRRRRRRRRRRRRRRRRRRRRR G 511 R 0 36.5233 Negative Pyruvate Kinase Deficiency GGGGGGGGGGGGGGGGGGGGGGGGGGGGGG R 518 S 0 2.93867 Positive Pyruvate Kinase Deficiency RKRKRKSRTTNKPPKEPPPHEPEEDEEPEP R 531 C 0 3.94415 Positive Pyruvate Kinase Deficiency RLRLRLRSMLLLNEVARRREANRAKSRKLR R 532 Q 0 14.4345 Negative Pyruvate Kinase Deficiency RRRRRRRRRRRRRRRRRRRRRRRRRRRRRR R 532 W 0 22.3141 Negative Pyruvate Kinase Deficiency RRRRRRRRRRRRRRRRRRRRRRRRRRRRRR D 550 V 0 12.3578 Negative Pyruvate Kinase Deficiency DDDDDDDDDDDDDDDNDDDDDADDDDDDDE V 552 M 0 7.38517 Intermediate Pyruvate Kinase Deficiency VVVVVVVVVAVVLLVIIIIVIIVYIIVVYV G 557 A 0 22.0523 Negative Pyruvate Kinase Deficiency GGGGGGGGGGGGGGGGGGGGGGGGGGGGGG R 559 G 0 6.55002 Intermediate Pyruvate Kinase Deficiency RRRRRRRRRRRRRKKKRRRTVKKKAARKKK N 566 K 0 26.0618 Negative Pyruvate Kinase Deficiency NNNNNNNNNNNNNNNNNNNTSNNNNNNNNN